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همه ویدیو ها

  • Stem Cell Embryoid Body Formation

    652 بازدید

    ( http://www.abnova.com ) - This video shows a novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel. [Reference: Biomacromolecules. 2007 Sep;8(9):2746-52]. More videos at Abnova http://www.abnova.com

  • Dot Blot

    101 بازدید

    ( http://www.abnova.com ) - Dot blot is a technique can be used as a semiqualitative method for rapid screening of a large number of samples. It is for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane. More videos at Abnova http://www.abnova.com

  • Cell Counting Attached cell

    127 بازدید

    ( http://www.abnova.com ) - This episode of the cell counting series demonstrates the handling of attached cell before counting. Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope. More videos at Abnova http://www.abnova.com

  • MTT Assay

    1,511 بازدید

    ( http://www.abnova.com ) - A demonstration on the procedure of using MTT assay to assess the viability and the proliferation of regular cells with absorbance detection at 595nm is shown. This colorimetric assay measures the reduction of yellow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) by mitochondrial succinate dehydrogenase. More videos at Abnova http://www.abnova.com

  • Plasmid DNA Extraction Miniprep

    220 بازدید

    ( http://www.abnova.com ) - Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes Gigaprep, Megaprep, and Midiprep. More videos at Abnova http://www.abnova.com

  • DNA Extraction from Mouse Tail Manual

    237 بازدید

    ( http://www.abnova.com ) - DNA extraction is a routine procedure to collect DNA for subsequent molecular analysis. This video shows you how to extract DNA from mouse tails. There are three basic steps: (i) break cells by lysis buffer, (ii) digest protein and remove contamination by proteinase, (iii) precipitating the DNA. More videos at Abnova http://www.abnova.com

  • Gel Extraction

    512 بازدید

    ( http://www.abnova.com ) - Gel extraction is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. Three basic steps are involved: slicing the bands of interest on UV light exposure, isolating the DNA from those bands, and removing the accompanying salts and stain. More videos at Abnova http://www.abnova.com

  • mRNA Extraction from Total RNA

    108 بازدید

    ( http://www.abnova.com ) - Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA. More videos at Abnova http://www.abnova.com

  • Nuclear and Cytoplasmic Protein Extraction

    43 بازدید

    ( http://www.abnova.com ) - Subcellular fractionation is extremely useful for assessing protein localization. This protocol shows how to separate and prepare of cytoplasmic and nuclear extracts from mammalian cultured cells. More videos at Abnova http://www.abnova.com

  • Plasmid DNA Extraction CsCI Isolation

    73 بازدید

    ( http://www.abnova.com ) - CsCI isolation is a widely used method for isolating highly pure circular plasmid DNA in relatively high yield from bacterial cells. This video demonstrates the procedures for CsCI isolation method. More videos at Abnova http://www.abnova.com

  • Plasmid DNA Extraction Gigaprep

    133 بازدید

    ( http://www.abnova.com ) - Gigaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 2.5~5 L of LB broth and the expected DNA yield is 7.5~10 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes megaprep, midiprep, and miniprep. More videos at Abnova http://www.abnova.com

  • Plasmid DNA Extraction Megaprep

    51 بازدید

    ( http://www.abnova.com ) - Megaprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 500 ml~2.5 L of LB broth and the expected DNA yield is 1.5~2.5 mg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, midiprep, and miniprep More videos at Abnova http://www.abnova.com

  • Plasmid DNA Extraction Midiprep

    84 بازدید

    ( http://www.abnova.com ) - Midiprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 15-25 ml of LB broth and the expected DNA yield is 100-350 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and miniprep. More videos at Abnova http://www.abnova.com

  • Total IgG (Human) ELISA Kit

    157 بازدید

    ( http://www.abnova.com ) - Total IgG (Human) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of total human IgG in human serum and plasma. This video shows the procedures of the kit. More videos at Abnova http://www.abnova.com

  • Raw Cell Transmigration

    102 بازدید

    ( http://www.abnova.com ) - The ability of cell migration is commonly used for the evaluation of cell invasion or wound repair. Using boyden chamber is convenient for the assessment of cell's responses toward genetic manipulation or chemo attraction treatment. More videos at Abnova http://www.abnova.com

  • Protein Quantification Coomassie Blue R CBR

    103 بازدید

    ( http://www.abnova.com ) - Coomassie Blue R (CBR) is sensitive for protein detection in PAGE gels, and protein can be directly quantitated by colorimetric. Coomassie Blue dye binds to peptides in an acidic medium. The color change of this assay is measured at 595 or 600 nm. We calculate the protein concentration using the formula created by standards. This AbVideo displays the procedure of protein quantification by using CBR. More videos at Abnova http://www.abnova.com

  • Protein Quantification BCA Assay

    198 بازدید

    ( http://www.abnova.com ) - The bicinchoninic acid assay (BCA Assay) is a biochemical assay for determining the total level of protein in a solution, similar to Bradford protein assay. The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques. More videos at Abnova http://www.abnova.com

  • Plaque Assay for Influenza Virus

    190 بازدید

    ( http://www.abnova.com ) - The plaque assay is a widely used approach for purifying a clonal population of virus and determining the viral titers (the lowest concentration of virus that results in infection). This video shows the procedure of plaque assay for influenza virus. More videos at Abnova http://www.abnova.com

  • Mycoplasma Assay

    84 بازدید

    ( http://www.abnova.com ) - Mycoplasma is highly infectious and cross-contamination commonly occurs when new cells are introduced into laboratories. We use PCR-based method for detection of Mycoplasma weekly. It is a highly sensitive, specific and rapid method to detect mycoplasma contamination in cell cultures. More videos at Abnova http://www.abnova.com

  • Mouse Genotyping

    78 بازدید

    ( http://www.abnova.com ) - Genotyping is the process of determining the genes (genotype) of an individual by examining its DNA with the use of biological assays (ex: PCR, DNA sequencing). Three steps to type the mouse genotype : (i) isolate the genomic DNA from mouse tails; (ii) PCR to amplify with specific primers; (iii) electrophoresis to determine the genotype. More videos at Abnova ( http://www.abnova.com ).

  • Migration Assay

    378 بازدید

    ( http://www.abnova.com ) - The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted. More videos at Abnova http://www.abnova.com

  • Luciferase Assay

    65 بازدید

    ( http://www.abnova.com ) - Firefly luciferase is widely used as a reporter to study gene expression and other cellular events. The Luciferase Assay is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Through the assay, light is produced by converting the chemical energy of luciferin oxidation and can be measured by a luminometer. More videos at Abnova http://www.abnova.com

  • Invasion Assay

    108 بازدید

    ( http://www.abnova.com ) - Cell invasion assay is designed to accelerate the screening process for compounds that influence cell migration through extracellular matrices. Matrigel (BD), extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, resembles the extracellular matrices is used by cell biologists as a substrate in cell invasion assay. More videos at Abnova http://www.abnova.com

  • FSH ELISA Kit

    133 بازدید

    ( http://www.abnova.com ) - FSH ELISA Kit is a solid-phase enzyme immunoassay for the quantitative detection of FSH. This video shows the procedures of the kit. More videos at Abnova http://www.abnova.com

  • Clonogenic Assay

    177 بازدید

    ( http://www.abnova.com ) - Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate. More videos at Abnova ( http://www.abnova.com ).

  • Cell Proliferation Assay WST-1

    324 بازدید

    ( http://www.abnova.com ) - The Cell Proliferation Reagent WST-1 is a clear, slightly red, ready-to-use solution, containing WST-1, a tetrazolium salt. By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of 440 nm. More videos at Abnova http://www.abnova.com

  • Cell Proliferation Assay MTS

    107 بازدید

    ( http://www.abnova.com ) - CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound (MTS). The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at 490nm. More videos at Abnova http://www.abnova.com

  • Antibody Conjugation - Biotinylation

    64 بازدید

    ( http://www.abnova.com ) - Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. A biotin/streptavidin (avidin) system offers low background, enhanced sensitivity and the ability to detect minute amounts of antigens. More videos at Abnova http://www.abnova.com

  • Lactate Dehydrogenase Assay Kit

    195 بازدید

    ( http://www.abnova.com ) - Lactate Dehydrogenase Assay Kit is a colorimetric kinetic determination of lactate dehydrogenase activity. Lactate Dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. This video shows the procedures of the kit. More videos at Abnova http://www.abnova.com

  • Plasmid DNA Extraction Alkaline Lysis Method

    66 بازدید

    ( http://www.abnova.com ) - Alkaline lysis is one of the most generally useful methods for isolating circular plasmid DNA from bacterial cells. This video demonstrates the procedures for alkaline lysis method. More videos at Abnova http://www.abnova.com

  • RNA Extraction by TRIzol

    278 بازدید

    ( http://www.abnova.com ) - TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol. More videos at Abnova ( http://www.abnova.com ).

  • RNA Extraction from Cell Culture

    98 بازدید

    ( http://www.abnova.com ) - Extracting RNA from cultured cells has traditionally been a highly repetitive method, and this AbVideo indicates the steps on how to do it. The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology. More videos at Abnova http://www.abnova.com